Before serum samples are tested
using the
SPOT Test
technology they are first
pre-treated to enhance accuracy and reliability. These
immunosorbant procedures significantly lower the
concentration of competing antibodies.
IgG
INTERFERENCE:
Upon exposure to antigen, the body
produces antibody (IgE and IgG) with IgG being produced in
larger quantities. This circulating IgG interferes with
the assay. To counter this problem all serum samples are
first absorbed with Staphylococcal protein A extracted
from the cell wall of S. aureus. This protein has
a strong binding affinity to the Fc portion of
the IgG molecule thus removing it from the serum.
NON-SPECIFIC
IgE INTERFERENCE:
The high levels of allergen
non-specific IgE are usually caused by parasitic
infections in animals. Allergen non-specific IgE competes
with allergen specific IgE for binding, thus skewing the
results. This problem is resolved by absorbing serum
samples with helmenthes antigens. Helmenthes are one of
the most common antigens responsible for high IgE levels.
The effect of this absorption is to lower the oeverall
concentration of interfering allergen non-specific IgE.
